Phasins, Multifaceted Polyhydroxyalkanoate Granule-Associated Proteins
نویسندگان
چکیده
منابع مشابه
Polyhydroxyalkanoate‐associated phasins as phylogenetically heterogeneous, multipurpose proteins
Polyhydroxyalkanoates (PHAs) are natural polyesters of increasing biotechnological importance that are synthesized by many prokaryotic organisms as carbon and energy storage compounds in limiting growth conditions. PHAs accumulate intracellularly in form of inclusion bodies that are covered with a proteinaceous surface layer (granule-associated proteins or GAPs) conforming a network-like surfac...
متن کاملLocalization of poly(3-hydroxybutyrate) (PHB) granule-associated proteins during PHB granule formation and identification of two new phasins, PhaP6 and PhaP7, in Ralstonia eutropha H16.
Poly(3-hydroxybutyrate) (PHB) granules are covered by a surface layer consisting of mainly phasins and other PHB granule-associated proteins (PGAPs). Phasins are small amphiphilic proteins that determine the number and size of accumulated PHB granules. Five phasin proteins (PhaP1 to PhaP5) are known for Ralstonia eutropha. In this study, we identified three additional potential phasin genes (H1...
متن کاملPolyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium.
Polyhydroxyalkanoic acids (PHA) are carbon and energy storage polymers that accumulate in inclusion bodies in many bacteria and archaea in response to environmental conditions. This work presents the results of a study of PHA inclusion body-associated proteins and an analysis of their coding region in Bacillus megaterium 11561. A 7, 917-bp fragment of DNA was cloned and shown to carry a 4,104-b...
متن کاملOverexpression and characterization of medium-chain-length polyhydroxyalkanoate granule bound polymerases from Pseudomonas putida GPo1
BACKGROUND Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two diffe...
متن کاملMolecular characterization of a regulatory protein (PhaR) involved in PHA biosynthesis
Phasins (PhaP) are polyhydroxyalkanoate (PHA) granule-associated proteins that positively affect the PHA synthesis. In the heterologous expression experiment in Escherichia coli, introduction of only phaP caused overproduction of PhaP, and apparent repression of PhaP production was observed in the presence of phaR, which is located in the downstream region of phaP. It indicates that PhaR seems ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2016
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01161-16